Course description:

The fastest and most gentle 3D live-cell imaging on the Carl Zeiss Lattice Lightsheet 7, used for tracking the trafficking of fluorescently labeled proteins in living cells, will be complemented by FRAP on the Nikon iLas 2 ring-TIRF/FRAP and Nikon CSU-W1, as well as by FCS on the Leica TCS SP8 WLL SMD-FLIM for measuring protein diffusion in the plasma membrane of living cells. Finally, model systems of molecular motors walking along microtubules, visualized using the Nikon Ti-E H-TIRF, will demonstrate the benefits of single-molecule approaches for studying molecular motion.

This course includes theoretical and hands-on training in four complementary microscopy approaches for visualizing and measuring the lateral movement of molecules in living cells or model systems.

The microscopy techniques covered in the course:

• Lattice light-sheet microscopy (LLS)
• Fluorescence recovery after photobleaching (FRAP)
• Fluorescence correlation spectroscopy (FCS)
• Single-particle tracking (SPT)

Registration and more information: