Technologies:

• Advanced light microscopy
• Fluorescence microscopy
• Super-resolution microscopy
• Electron microscopy
• Correlative light and electron microscopy
• Sample preparation
• Neuroscience imaging
• Magnetic resonance
• Magnetic particle imaging
• Image analysis and development of new methods

Specialities and expertise of the Node

The Light Microscopy (LM) core facility at IMG Czech Acad. Sci. is focused on cutting-edge microscopy techniques particularly on time-gated STED microscopy. It provides strong support in image analysis and processing, more specifically in image reconstruction and deconvolution, image registration, volume reconstruction, image segmentation, tracking, colocalization analysis, analysis of photokinetic experiments, 3D visualization and mathematical modeling and simulations of biological processes.

The Electron Microscopy (EM) core facility at IMG Czech Acad. Sci. has a long expertise in the development and optimization of sample preparation techniques for electron microscopy, e.g. development of a new system for simultaneous immunolabeling of multiple molecular targets, a special technique for combination of 3D pre-embedding immunolabeling and 2D on-section immunolabeling, a statistical tool for evaluation of clustering and colocalization of immunolabeling in EM.

The Imaging core facility at BIOCEV focuses on fluorescence super-resolution imaging, functional imaging, two-photon imaging and scanning electron microscopy. The strength in image analysis is especially in fluorescence lifetime imaging data analysis and in spatio-temporal correlation techniques, for which unique data analysis modules were developed.

Image analysis and stereology is an area of expertise of the IPHYS BioImaging Facility. It provides development of new methods and SW modules (in ImageJ, Amira or Ellipse SW environment) in the field of image processing and analysis, stereology and spatial statistics.

Imaging facility of the Institute of Experimental Botany (IFIEB) focuses on microscopy in plant research. IFIEB provides high-end instrumentation and expertise for non-invasive in vivo fluorescence microscopy in high resolution. Plant-optimized vertical-stage SR microscope with root growth tracking algorithm allows long-term imaging of seedlings growing in their natural orientation.

Technologies:

• Advanced light microscopy
• Fluorescence microscopy
• Super-resolution microscopy
• Electron microscopy
• Correlative light and electron microscopy
• Sample preparation
• Neuroscience imaging
• Magnetic resonance
• Magnetic particle imaging
• Image analysis and development of new methods

Specialities and expertise of the Node

The Euro-BioImaging Node Candidate in Brno consists of two parts. The Medical Imaging part is formed by two closely collaborating facilities. One is focused on animal ultra-high field MR imaging and spectroscopy (9.4T), the other on human MR imaging (3T) accompanied with electrophysiological techniques. Together, these facilities enable translational research and offer a complex portfolio of MRI techniques including multimodal approaches (e.g. simultaneous EEG-fMRI) and human hyper-scanning (fMRI with two participants measured simultaneously in two scanners). The Advanced Light Microscopy part consist of central core facility Cellular Imaging (CELLIM), the Experimental Biophotonics Facility and several closely cooperating laboratories offering together access to equipment, training and image analysis tools. The light microscopy is further specialized in imaging of plant systems, mammalian germs cells, stem cells and embryos, and development of image analysis tools. Additionally, the Node is able to offer holographic incoherent quantitative phase imaging in correlation with laser scanning confocal microscopy.

Technologies:

• MRI/MRS or micro-MRI/MRS
• Optical imaging (OI)
• CT or micro-CT
• PET/CT or micro-PET/CT
• SPECT/CT or micro-SPECT/CT
• US or micro-US
• Photoacoustic Imaging (PAI)*

Specialities and expertise of the Node

CAPI is the only multimodal imaging center in the Czech Republic, offering hybrid imaging with co-registration of different modalities for single animals and some of the most innovative methods such as Magnetic Particle Imaging, High Frequency Ultrasound combined with Photoacoustic Imaging, and Spectral X-Ray Imaging.